研究業績
原著論文
2023年
-
Ganglong Yang, Chenyang Zuo, Yuxiang Lin, Xiaoman Zhou, Piaopiao Wen, Chairui Zhang, Han Xiao, Meichen Jiang, Morihisa Fujita, Xiao-Dong Gao, Fangmeng Fu:
Comprehensive proteome, phosphoproteome and kinome characterization of luminal A breast cancer.
Front. Oncol., 13:1127446 (2023).
-
Shu-Ya Cao, Yi-Shi Liu, Xiao-Dong Gao, Taroh Kinoshita, and Morihisa Fujita*:
A lipid scramblase TMEM41B is involved in the processing and transport of GPI-anchored proteins.
J. Biochem., 174(2):109-123 (2023).
-
Yi-Shi Liu, Yicheng Wang, Xiaoman Zhou, Linpei Zhang, Ganglong Yang, Xiao-Dong Gao, Yoshiko Murakami, Morihisa Fujita*, and Taroh Kinoshita:
Accumulated precursors of specific GPI-anchored proteins upregulate GPI biosynthesis with ARV1.
J. Cell Biol., 222(5):e202208159 (2023).
2022年
-
Shijiao Huang, Yoshimi Haga, Jie Li, Jianchao Zhang, Hye Kyong Kweon, Junichi Seino, Hiroto Hirayama, Morihisa Fujita, Kelley W Moremen, Philip Andrews, Tadashi Suzuki, and Yanzhuang Wang:
Mitotic phosphorylation inhibits the Golgi mannosidase MAN1A1.
Cell Rep., 41(8):111679 (2022).
-
Piaopiao Wen, Jingru Chen, Chenyang Zuo, Xiao-Dong Gao, Morihisa Fujita*, and Ganglong Yang:
Proteome and glycoproteome analyses reveal the protein N-linked glycosylation specificity of STT3A and STT3B.
Cells, 11(18):2775 (2022).
-
Tetsuya Hirata, Jing Yang, Seita Tomida, Yuko Tokoro, Taroh Kinoshita, Morihisa Fujita, Yasuhiko Kizuka:
ER entry pathway and glycosylation of GPI-anchored proteins are determined by N-terminal signal sequence and C-terminal GPI-attachment sequence.
J. Biol. Chem., in press (2022).
-
Yicheng Wang, Anant K. Menon, Yuta Maki, Yi-Shi Liu, Yugo Iwasaki, Morihisa Fujita, Paula A. Guerrero, Daniel Varon Silva, Peter H. Seeberger, Yoshiko Murakami, and Taroh Kinoshita:
Genome-wide CRISPR screen reveals CLPTM1L as a lipid scramblase required for efficient glycosylphosphatidylinositol biosynthesis.
Proc. Natl. Acad. Sci. USA, 119(14):e2115083119 (2022).
-
Smrithi Salian, Xin-Yu Guo, Yoshiko Murakami, Taroh Kinoshita, Parneet Kaur, Anju Shukla, Katta M Girisha, Morihisa Fujita, Philippe M Campeau:
C18orf32 loss-of-function is associated with a neurodevelopmental disorder with hypotonia and contractures.
Hum. Genet., 141(8):1423-1429 (2022).
2021年
-
Si-Si Liu, Fei Jin, Yi-Shi Liu, Yoshiko Murakami, Yukihiko Sugita, Takayuki Kato, Xiao-Dong Gao, Taroh Kinoshita, Motoyuki Hattori, and Morihisa Fujita*:
Functional analysis of the GPI transamidase complex by screening for amino acid mutations in each subunit.
Molecules, 26(18):5462 (2021).
-
Yi-Fan Huang, Shuji Mizumoto, and Morihisa Fujita*:
Novel insight into glycosaminoglycan biosynthesis based on gene expression profiles.
Frontiers Cell Dev. Biol., 9: 709018 (2021).
-
Si-Si Liu, Yi-Shi Liu, Xin-Yu Guo, Yoshiko Murakami, Ganglong Yang, Xiao-Dong Gao, Taroh Kinoshita and Morihisa Fujita*:
A knockout cell library of GPI biosynthetic genes for functional studies of GPI-anchored proteins.
Commun. Biol., 4: 777 (2021).
-
Yi-Fan Huang, Kazuhiro Aoki, Sachiko Akase, Mayumi Ishihara, Yi-Shi Liu, Ganglong Yang, Yasuhiko Kizuka, Shuji Mizumoto, Michael Tiemeyer, Xiao-Dong Gao, Kiyoko F. Aoki-Kinoshita, and Morihisa Fujita*:
Global mapping of glycosylation pathways in human-derived cells.
Dev. Cell, 56(8):1195-1209 (2021).
-
Xin-Yu Guo, Xiao-Dong Gao, and Morihisa Fujita*:
Sulfation of a FLAG tag mediated by SLC35B2 and TPST2 affects antibody recognition.
PLoS ONE, 16(5):e0250805 (2021).
-
Jing Yang, Tetsuya Hirata, Yi-Shi Liu, Xin-Yu Guo, Xiao-Dong Gao, Taroh Kinoshita, and Morihisa Fujita*:
Human SND2 mediates ER targeting of GPI-anchored proteins with low hydrophobic GPI-attachment signals.
FEBS Lett., 595(11):1542-1558 (2021).
-
Ji-Xiong Leng, Wei-Wei Ren, Li Y, Ganglong Yang, Xiao-Dong Gao, and Morihisa Fujita*:
Cell engineering for the production of hybrid-type N-glycans in HEK293 cells.
J. Biochem., 170(1):139-151 (2021).
-
Yi-Shi Liu, Emmanuel Matabaro, Xiao-Dong Gao, and Morihisa Fujita*:
Selecting cells expressing high levels of recombinant proteins using the GPI-anchored protein with selenocysteine system.
J. Biosci. Bioeng., 131(3):225-233 (2021).
2020年
-
Wenyan Chen, Rong Wang, Dan Li, Chenyang Zuo, Piaopiao Wen, Haili Liu, Yongquan Chen, Morihisa Fujita, Zhimeng Wu, and Ganglong Yang:
Comprehensive Analysis of the Glycome and Glycoproteome of Bovine Milk-Derived Exosomes.
J. Agric. Food Chem., 68(45):12692-12701 (2020).
-
Xin-Yu Guo, Yi-Shi Liu, Xiao-Dong Gao, Taroh Kinoshita, and Morihisa Fujita*:
Calnexin mediates the maturation of GPI-anchors through ER retention.
J. Biol. Chem., 295(48):16393-16410 (2020).
-
Ran Cao, Tian-Chan Zhang, Ya-Ran Chen, Cui Cao, Huan Chen, Yi-Fan Huang, Morihisa Fujita, Li Liu, and Josef Voglmeir:
Aberration of Serum and Tissue N-Glycans in Mouse β1,4-GalT1 Y286L Mutant Variants.
Glycoconj. J., 37(6):767-775 (2020).
-
Gun-Hee Lee, Morihisa Fujita, Hideki Nakanishi, Haruhiko Miyata, Masahito Ikawa, Yusuke Maeda, Yoshiko Murakami, and Taroh Kinoshita:
PGAP6, a GPI-specific phospholipase A2, has narrow substrate specificity against GPI-anchored proteins.
J. Biol. Chem., 295(42): 14501-14509 (2020).
-
Shen-Bao Zhao, Neta Dean, Xiao-Dong Gao, and Morihisa Fujita*:
MON2 Guides Wntless Transport to the Golgi through Recycling Endosomes.
Cell Struct. Funct., 45(1): 77-92 (2020).
-
Yicheng Wang, Yusuke Maeda, Yi-Shi Liu, Yoko Takada, Akinori Ninomiya, Tetsuya Hirata, Morihisa Fujita, Yoshiko Murakami, and Taroh Kinoshita:
Cross-talks of glycosylphosphatidylinositol biosynthesis with glycosphingolipid biosynthesis and ER-associated degradation.
Nat. Commun., 11(1): 860 (2020).
2019年
-
Wei-Wei Ren, Ze-Cheng Jin, Weijie Dong, Toshihiko Kitajima, Xiao-Dong Gao, and Morihisa Fujita*:
Glycoengineering of HEK293 Cells to Produce High-Mannose-Type N-Glycan Structures.
J. Biochem., 166(3): 245-258 (2019).
-
Shen-Bao Zhao, Yasuyuki Suda, Hideki Nakanishi, Ning Wang, Takehiko Yoko-o, Xiao-Dong Gao, and Morihisa Fujita*:
Yeast Dop1 is required for glycosyltransferase retrieval from the trans-Golgi network.
Biochim. Biophys. Acta – General Subjects, 1863: 1147-1157 (2019).
-
Emmanuel Osei Mensah, Xin-Yu Guo, Xiao-Dong Gao, and Morihisa Fujita*:
Establishment of DHFR-deficient HEK293 cells for high yield of therapeutic glycoproteins.
J. Biosci. Bioeng., 128(4): 487-494 (2019).
-
Yicheng Wang, Tetsuya Hirata, Yusuke Maeda, Yoshiko Murakami, Morihisa Fujita, Taroh Kinoshita:
Free, unlinked glycosylphosphatidylinositols on mammalian cell surfaces revisited.
J. Biol. Chem., 294(13): 5038-5049 (2019).
2018年
-
Xin-Xin Xu, Sheng-Tao Li, Ning Wang, Toshihiko Kitajima, Takehiko Yoko-o, Morihisa Fujita, Hideki Nakanishi, and Xiao-Dong Gao:
Structural and functional analysis of Alg1 beta-1,4 mannosyltransferase reveals the physiological importance of its membrane topology.
Glycobiology, 28(10): 741-753 (2018).
-
Diane Mutumwinka, Shen-Bao Zhao, Yi-Shi Liu, Emmanuel Osei Mensah, Xiao-Dong Gao, and Morihisa Fujita*:
PiggyBac-based screening identified BEM4 as a suppressor to rescue growth defects in och1-disrupted yeast cells.
Biosci. Biotechnol. Biochem., 82(9): 1497-1507 (2018).
-
Ze-Cheng Jin, Toshihiko Kitajima, Weijie Dong, Yi-Fan Huang, Wei-Wei Ren, Feng Guan, Yasunori Chiba, Xiao-Dong Gao, and Morihisa Fujita*:
Genetic disruption of multiple α1,2-mannosidases generates mammalian cells producing recombinant proteins with high-mannose-type N-glycans.
J. Biol. Chem., 293(15): 5572-5584 (2018).
-
Tetsuya Hirata, Sushil K. Mishra, Shota Nakamura, Kazunobu Saito, Daisuke Motooka, Yoko Takada, Noriyuki Kanzawa, Yoshiko Murakami, Yusuke Maeda, Morihisa Fujita, Yoshiki Yamaguchi, and Taroh Kinoshita:
Identification of a Golgi GPI-N-acetylgalactosamine transferase with tandem transmembrane regions in the catalytic domain.
Nat. Commun., 9(1): 405 (2018).
-
Yi-Shi Liu, Xin-Yu Guo, Tetsuya Hirata, Yao Rong, Daisuke Motooka, Toshihiko Kitajima, Yoshiko Murakami, Xiao-Dong Gao, Shota Nakamura, Taroh Kinoshita, and Morihisa Fujita*:
N-Glycan dependent protein folding and ER retention regulates GPI-anchor processing.
J. Cell Biol., 217(2): 585-599 (2018).
-
Toshihiko Kitajima, Wei Xue, Yi-Shi Liu, Chu-Di Wang, Si-Si Liu, Morihisa Fujita, Xiao-Dong Gao:
Construction of green fluorescence protein mutant to monitor STT3B-dependent N-glycosylation.,
FEBS J., 285(5): 915-928 (2018).
-
Sheng-Tao Li, Ning Wang, Xin-Xin Xu, Morihisa Fujita, Hideki Nakanishi, Toshihiko Kitajima, Neta Dean, Xiao-Dong Gao:
Alternative routes for synthesis of N-linked glycans by Alg2 mannosyltransferase.,
FASEB J., 32(5): 2492-2506 (2018).
2017年
-
Emmanuel Matabaro, Zeng’an He, Yi-Shi Liu, Hui-Jie Zhang, Xiao-Dong Gao, and Morihisa Fujita*:
Molecular switching system using glycosylphosphatidylinositol to select cells highly expressing recombinant proteins.,
Sci. Rep., 7(1): 4033 (2017).
-
Ting Yan, Huijie Zhang, Dandi Huang, Shini Feng, Morihisa Fujita, and Xiao-Dong Gao:
Chitosan-Functionalized Graphene Oxide as a Potential Immunoadjuvant.,
Nanomaterials, 7(3): E59 (2017).
-
Huijie Zhang, Ting Yan, Sha Xu, Shini Feng, Dandi Huang, Morihisa Fujita, and Xiao-Dong Gao:
Graphene oxide-chitosan nanocomposites for intracellular delivery of immunostimulatory CpG oligodeoxynucleotides.,
Mater. Sci. Eng. C Mater. Biol. Appl., 73:144-151 (2017).
-
Masamishi Nagae, Dorothee Liebschner, Yusuke Yamada, Kana Morita-Matsumoto, Naohiro Matsugaki, Toshiya Senda, Morihisa Fujita, Taroh Kinoshita, and Yoshiki Yamaguchi:
Crystallographic analysis of murine p24γ2 Golgi dynamics domain.,
Proteins, 85(4):764-770 (2017).
2016年
-
Gun-Hee Lee#, Morihisa Fujita#, Katsuyoshi Takaoka, Yoshiko Murakami, Yoshitaka Fujihara, Noriyuki Kanzawa, Kei-ichi Murakami., Eriko Kajikawa, Yoko Takada, Kazunobu Saito, Masahito Ikawa, Hiroshi Hamada, Yusuke Maeda, and Taroh Kinoshita (#共同筆頭著者):
A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling in embryos by shedding CRIPTO.,
J. Cell Biol., 215(5):705-718 (2016).
-
Masamichi Nagae, Tetsuya Hirata, Kana Morita-Matsumoto, Romina Theiler, Morihisa Fujita, Taroh Kinoshita, and Yoshiki Yamaguchi:
3D Structure and Interaction of p24β and p24δ Golgi Dynamics Domains: Implication for p24 Complex Formation and Cargo Transport.,
J. Mol. Biol., 428(20):4087-4099 (2016).
2015年
-
Yao Rong, Shota Nakamura, Tetsuya Hirata, Daisuke Motooka, Yi-Shi Liu, Zeng-An He, Xiao-Dong Gao, Yusuke Maeda, Taroh Kinoshita, and Morihisa Fujita*:
Genome-Wide Screening of Genes Required for Glycosylphosphatidylinositol Biosynthesis.,
PLoS ONE, 10: e0138553 (2015).
-
Tetsuya Hirata#, Morihisa Fujita#, *, Shota Nakamura, Kazuyoshi Gotoh, Daisuke Motooka, Yoshiko Murakami, Yusuke Maeda, and Taroh Kinoshita*:
Post-Golgi anterograde transport requires GARP-dependent endosome-to-TGN retrograde transport.,
Mol. Biol. Cell, 26: 3071-84 (2015).
トップへ戻る
2014年以前
-
Romina Theiler, Morihisa Fujita, Masamichi Nagae, Yoshiki Yamaguchi, Yusuke Maeda, and Taroh Kinoshita:
The α-helical region in p24γ2 subunit of p24 protein cargo receptor is pivotal for the recognition and transport of glycosylphosphatidylinositol-anchored proteins.,
J. Biol. Chem., 289: 16835-43 (2014).
-
Tetsuya Hirata, Morihisa Fujita, Noriyuki Kanazawa, Yoshiko Murakami, Yusuke Maeda, and Taroh Kinoshita:
Glycosylphosphatidylinositol mannosyltransferase II is the rate-limiting enzyme in glycosylphosphatidylinositol biosynthesis under limited dolichol-phosphate mannose availability.,
J. Biochem., 154: 257-264 (2013).
-
Noriyuki Kanzawa, Nobuyuki Shimozawa, Ronald J. A. Wanders, Kazutaka Ikeda, Yoshiko Murakami, Hans R. Waterham, Satoru Mukai, Morihisa Fujita, Yusuke Maeda, Ryo Taguchi, Yukio Fujiki and Taroh Kinoshita:
Defective lipid remodeling of GPI anchors in peroxisomal disorders, Zellweger syndrome, and rhizomelic chondrodysplasia punctata.,
J. Lipid Res., 53:653-663 (2012).
-
Morihisa Fujita, Reika Watanabe, Nina Jaensch, Maria Romanova-Michaelides, Tadashi Satoh, Masaki Kato, Howard Riezman, Yoshiki Yamaguchi, Yusuke Maeda, and Taroh Kinoshita:
Sorting of GPI-anchored proteins into ER exit sites by p24 proteins is dependent on remodeled GPI.,
J. Cell Biol., 194: 61-75 (2011).
-
Yuri Nakano, Morihisa Fujita, Kazutoyo Ogino, Taroh Kinoshita, Yoichi Oda, and Hiromi Hirata:
Biogenesis of GPI-anchored proteins is essential for surface expression of sodium channels in zebrafish Rohon-Beard neurons to respond to mechanosensory stimulation.,
Development, 137: 1689-1698 (2010).
-
Atsuhiro Adachi, Fumi Kano, Takashi Tsuboi, Morihisa Fujita, Yusuke Maeda, and Masayuki Murata:
Golgi-associated GSK3{beta} regulates the sorting process of post-Golgi membrane trafficking.,
J. Cell Sci., 123: 3215-3225 (2010).
-
Morihisa Fujita, Yusuke Maeda, Moonjin Ra, Yoshiki Yamaguchi, Ryo Taguchi, and Taroh Kinoshita:
GPI glycan remodeling by PGAP5 regulates transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi.,
Cell, 139: 352-365 (2009).
-
Hiroto Hirayama, Morihisa Fujita, Takehiko Yoko-o, and Yoshifumi Jigami:
O-mannosylation is required for degradation of the endoplasmic reticulum-associated degradation substrate Gas1*p via the ubiquitin/proteasome pathway in Saccharomyces cerevisiae.,
J. Biochem., 143(4): 555-67 (2008).
-
Mariko Umemura, Morihisa Fujita, Takehiko Yoko-o, Akiyoshi Fukamizu, and Yoshifumi Jigami:
Saccharomyces cerevisiae CWH43 is involved in the remodeling of the lipid moiety of GPI anchors to ceramides.,
Mol. Biol. Cell, 18(11): 4304-16 (2007).
-
Yusuke Maeda, Yuko Tashima, Toshiaki Houjou, Morihisa Fujita, Takehiko Yoko-o, Yoshifumi Jigami , Ryo Taguchi, and Taroh Kinoshita:
Fatty acid remodeling of GPI-anchored proteins is required for their raft association.,
Mol. Biol. Cell, 18(4): 1497-506 (2007).
-
Morihisa Fujita, Mariko Umemura, Takehiko Yoko-o, and Yoshifumi Jigami:
PER1 is required for GPI-phospholipase A2 activity and involved in lipid remodeling of GPI-anchored proteins.,
Mol. Biol. Cell, 17(12): 5253-64 (2006).
-
Morihisa Fujita, Takehiko Yoko-o, and Yoshifumi Jigami:
Inositol deacylation by Bst1p is required for the quality control of glycosylphosphatidylinositol-anchored proteins.,
Mol. Biol. Cell, 17(2): 834-50 (2006).
-
Morihisa Fujita, Takehiko Yoko-o, Michiyo Okamoto, and Yoshifumi Jigami:
GPI7 involved in glycosylphosphatidylinositol biosynthesis is essential for yeast cell separation.,
J. Biol. Chem., 279(50): 51869-79 (2004).
トップへ戻る
英文総説
-
Yi-Shi Liu and Morihisa Fujita*:
Mammalian GPI-anchor modifications and the enzymes involved.,
Biochem. Soc. Trans., 48(3): 1129-1138 (2020).
-
Taroh Kinoshita and Morihisa Fujita:
Biosynthesis of GPI-anchored proteins: special emphasis on GPI lipid remodeling.,
J. Lipid Res. 57: 6-24 (2016).
-
Taroh Kinoshita, Yusuke Maeda and Morihisa Fujita:
Transport of glycosylphosphatidylinositol-anchored proteins from the endoplasmic reticulum.,
Biochim. Biophys. Acta – Mol. Cell Res., 1833: 2473-8 (2013).
-
Morihisa Fujita*:
Potential Roles of GPI-Anchor Remodeling in Protein Trafficking and Raft Association in Mammalian Cells.,
Trends Glycosci. Glycotech., 24: 244-257 (2012).
-
Morihisa Fujita* and Taroh Kinoshita:
GPI-anchor remodeling: Potential functions of GPI-anchors in intracellular trafficking and membrane dynamics.,
Biochim. Biophys. Acta, 1821 (8): 1050-1058 (2012).
-
Morihisa Fujita and Taroh Kinoshita:
Structural remodeling of GPI anchors during biosynthesis and after attachment to proteins.,
FEBS Lett., 584 (9): 1670-1677 (2010).
-
Yusuke Maeda, Morihisa Fujita, and Taroh Kinoshita:
GPI-Anchor: Update for Biosynthesis and Remodeling.,
Trends Glycosci. Glycotech., 22: 182-193 (2010).
-
Taroh Kinoshita, Morihisa Fujita, and Yusuke Maeda:
Biosynthesis, remodelling and functions of mammalian GPI-anchored proteins: recent progress.,
J. Biochem., 144(3): 287-94 (2008).
-
Morihisa Fujita and Yoshifumi Jigami:
Lipid remodeling of GPI-anchored proteins and its function.,
Biochim. Biophys. Acta, 1780(3): 410-20 (2008).
トップへ戻る
和文総説
-
藤田盛久
GPIアンカー型タンパク質の生合成・リモデリング機構, 生化学, 日本生化学会, Vol. 85, 985-995 (2013).
-
藤田盛久
糖脂質修飾が決めるタンパク質の選別輸送機構 GPIアンカーの構造変化によるタンパク質輸送・膜局在の制御, 化学と生物, 日本農芸化学会, Vol. 50, 564-566 (2012).
-
藤田盛久、木下タロウ
生体膜ダイナミクスにおけるGPIアンカーの機能, 細胞工学, 秀潤社, Vol. 30, 972-979 (2011).
-
藤田盛久、木下タロウ
GPI付加によるタンパク質選別輸送・局在の調節, 実験医学, 羊土社, Vol. 28, 3285-3291 (2010).
-
神澤範行、藤田盛久、木下タロウ
GPIアンカーの脂質・糖鎖リモデリング, 実験医学, 羊土社, Vol. 28, 1251-1256 (2010).
-
藤田盛久、前田裕輔、木下タロウ
GPIアンカー蛋白質のリモデリング機構, 蛋白質核酸酵素, Vol. 53, 1442-1447 (2008).
-
藤田盛久
タンパク質の品質管理機構 細胞内タンパク質生産工場の品質管理責任者の発見, 産総研TODAY Vol. 6, No. 3, p18-19 (2006).
トップへ戻る
著書
-
Wei-Wei Ren and Morihisa Fujita*:
Simple N-Glycan Profile Analysis Using Lectin Staining, Mass Spectrometry, and GlycoMaple.,
Methods Mol. Biol., 2557:691-707 (2023).
-
Sneha Sudha Komath, Morihisa Fujita, Gerald W. Hart, Michael A.J. Ferguson, and Taroh Kinoshita:
Glycosylphosphatidylinositol Anchors.,
Essentials of Glycobiology, 4th edition. Cold Spring Harbor Laboratory Press., Chapter 12 (2022).
-
Xin-Yu Guo and Morihisa Fujita*:
Glycosylphosphatidylinositol Anchors and Lipids.,
Comprehensive Glycoscience, 2nd edition Elsevier., 3: 103-116 (2021).
-
Si-Si Liu and Morihisa Fujita*:
Glycosylphosphatidylinositol Anchors.,
Encyclopedia of Biological Chemistry, second ed. Academic Press., 2: 663-671 (2021).
-
Morihisa Fujita*, Xiao-Dong Gao and Taroh Kinoshita:
Glycan-Mediated Protein Transport from the Endoplasmic Reticulum.,
Sugar Chains, Springer: 31 - 34 (2015).
-
Taroh Kinoshita and Morihisa Fujita:
Overview of GPI biosynthesis.,
The Enzymes, Academic Press, 26: 1-30 (2009).
-
地神芳文、藤田盛久
タンパク質の糖鎖修飾, 酵母のすべて, シュプリンガー・フェアラーク東京 (2007).
トップへ戻る
expand_less