Institute for Glyco-core Research (iGCORE)|
Faculty of Applied Biological Sciences | Graduate School of Natural Science and Technology |
United Graduate School of Agricultural Science
Glyco-biochemistry lab (Kizuka lab)


Original papers
Review papers

Original papers


  • Abe J., Takeda Y., Kikuma T., Kizuka Y., Kajiura H., Kajihara Y., Ito Y. (2023) Squaryl group-modified UDP analogs as inhibitors of the endoplasmic reticulum-resident folding sensor enzyme UGGT. Chem. Commun., 59, 2803-2806.
  • Ohkawa Y., Kitano M., Maeda K., Nakano M., Kanto N.,Kizuka Y., Seike M., Azuma A., Yamaguchi Y., Ookawara T., Miyoshi E., Taniguchi N. (2023) Core fucosylation is required for the secretion of and the enzymatic activity of SOD3 in non-small cell lung cancer cells. Antioxid. Redox Signal., in press.


  • Hirata T., Harada Y., Hirosawa K.M., Tokoro Y., Suzuki K.G.N., *Kizuka Y. (2022) N-Acetylglucosaminyltransferase-V (GnT-V)-enriched small extracellular vesicles mediate N-glycan remodeling in recipient cells. iScience, 26, 105747. *corresponding author
  • Tomida S., Nagae M.,*Kizuka Y. (2022) The stem region of α1,6-fucosyltransferase FUT8 is required for multimer formation but not catalytic activity. J. Biol. Chem., 298, 102676. *corresponding author
  • Hirata T., Yang J., Tomida S., Tokoro Y., Kinoshita T., Fujita M., *Kizuka Y., (2022) ER entry pathway and glycosylation of GPI-anchored proteins are determined by N-terminal signal sequence and C-terminal GPI-attachment sequence. J. Biol. Chem., 298, 102444. *corresponding author
  • Osada N., Nagae M., Nakano M., Hirata T., *Kizuka Y. (2022) Examination of differential glycoprotein preferences of N-acetylglucosaminyltransferase-IV isozymes a and b. J. Biol. Chem., 298, 1024000. *corresponding author
  • Hirata T., Takata M., Tokoro Y., Nakano M., *Kizuka Y. (2022) Shedding of N-acetylglucosaminyltransferase-V (GnT-V) is regulated by maturity of cellular N-glycan. Commun. Biol., 5, 743, *corresponding author
  • Nagae M., Hirata T., Tateno H., Mishra S.K., Manabe N., Osada N., Tokoro Y., Yamaguchi Y., Doerksen R.J., Shimizu T., *Kizuka Y. (2022) Discovery of a lectin domain that regulates enzyme activity in mouse N-acetylglucosaminyltransferase-IVa (MGAT4A). Commun. Biol., 5, 695. *corresponding author
  • Hirata T., Takekiyo T., Yoshimura Y., Tokoro Y., Ishizaki T., Kizuka Y., Kuroda K. (2022) Cryostorage of unstable N-acetylglucosaminyltransferase-V by synthetic zwitterions. RSC Adv., 12, 11628-1631.
  • Vibhute A.M., Tanaka H.N., Mishra S.K., Osuka R.F., Nagae M., Yonekawa C., Korekane H., Doerksen R.J., Ando H., *Kizuka Y. (2022) Structure-based design of UDP-GlcNAc analogs as candidate GnT-V inhibitors. BBA Gen. Subj. , 1866, 130118.*corresponding author
  • Hirata T., Kobayashi A., Furuse T., Yamada I., Tamura M., Tomita H., Tokoro Y., Ninomiya A., Fujihara Y., Ikawa M., Maeda Y., Murakami Y., *Kizuka Y., Kinoshita T. (2022) Loss of the N-acetylgalactosamine side chain of the GPI-anchor impairs bone formation and brain functions and accelerates the prion disease pathology. J. Biol. Chem.,101720. *corresponding author
  • Osuka RF., Hirata T., Nagae M., Nakano M., Shibata H., Okamoto R., *Kizuka Y. (2022) N-Acetylglucosaminyltransferase-V requires a specific non-catalytic luminal domain for its activity toward glycoprotein substrates. J. Biol. Chem., 101666. *corresponding author


  • Kawade H., Morise J., Mishra S.K., Tsujioka S., Oka S., *Kizuka Y. (2022) Tissue-specific regulation of HNK-1 biosynthesis by bisecting GlcNAc. Molecules, 26, 5176. *corresponding author
  • *Ohkawa Y., *Kizuka Y., Takata M., Ito E., Mishra S.K., Akatsuka H., Harada Y., Taniguchi N. (2021) Peptide sequence mapping around bisecting GlcNAc-bearing N-glycans in mouse brain. Int. J. Mol. Sci., 22, 8579. *equal contribution
  • Huang YF., Aoki K., Akase S., Ishihara M., Liu YS., Yang G., Kizuka Y.,Mizumoto S., Tiemeyer M., Gao XD., Aoki-Kinoshita KF., Fujita M. (2021) Global mapping of glycosylation pathways in human-derived cells. Dev. Cell, 56, 1195-1209
  • Kitano M., Kizuka Y., Sobajima T., Nakano M., Nakajima K., Misaki R., Itoyama S., Harada Y., Harada A., Miyoshi E., Taniguchi N. (2021) Rab11-mediated post-Golgi transport of the sialyltransferase ST3GAL4 suggests a new mechanism for regulating glycosylation. J. Biol. Chem.,296, 100354


  • Hirata T., Nagae M., Osuka R.F., Mishra S.K., Yamada M., *Kizuka Y. (2020) Recognition of glycan and protein substrates by N-acetylglucosaminyltransferase-V (GnT-V). BBA Gen. Subj., 1864, 129726. *corresponding author
  • Ma C., Takeuchi H., Hao H., Yonekawa C., Nakajima K., Nagae M., Okajima T., Haltiwanger R., *Kizuka Y. (2020) Differential labeling of glycoproteins with alkynyl fucose analogs. Int. J. Mol. Sci., 21, 6007. *corresponding author
  • Tomida S., Takata M., Hirata T., Nagae M., Nakano M., *Kizuka Y. (2020) The SH3 domain in the fucosyltransferase FUT8 controls FUT8 activity and localization and is essential for core fucosylation. J. Biol. Chem., 295, 7992-8004. *corresponding author


  • Hashimoto M., Hirata T., Yonekawa C., Takeichi K., Fukamizu A., Nakagawa T., *Kizuka Y. (2019) Region-specific upregulation of HNK-1 glycan in PRMT1-deficient brain. BBA Gen. Subj., 1864, 129509. *corresponding author
  • Nakano M., Mishra S.K., Tokoro Y., Sato K., Nakajima K., Yamaguchi Y., Taniguchi N., *Kizuka Y. (2019) Bisecting GlcNAc is a general suppressor of terminal modification of N-glycan. Mol. Cell. Proteomics, 18, 2044-2057. *corresponding author
  • Harada Y., Kizuka Y., Tokoro Y., Kondo K., Yagi H., Kato K., Inoue H., Taniguchi N., Maruyama I. (2019) N-glycome inheritance from cells to extracellular vesicles in B16 melanomas. FEBS Lett., 593, 942-951.
  • Harada Y., Suzuki T., Fukushige T., Kizuka Y., Yagi H., Yamamoto M., Kondo K., Inoue H., Kato K., Taniguchi N., Kanekura T., Dohmae N., Maruyama I. (2019) Generation of the heterogeneity of extracellular vesicles by membrane organization and sorting machineries. BBA Gen. Subj., 1863, 681-691.


  • *Nagae M., *Kizuka Y., Mihara E., Kitago Y., Hanashima S., Ito Y., Takagi J., Taniguchi N., Yamaguchi Y. (2018) Structure and mechanism of cancer-associated N-acetylglucosaminyltransferase-V (GnT-V). Nat. Commun., 9, 3380. *equal contribution
  • Imamaki R., Ogawa K., Kizuka Y., Komi Y., Kojima S., Kotani N., Honke K., Honda T., Taniguchi N., Kitazume S. (2018) Glycosylation controls cooperative PECAM-VEGFR2-β3 integrin functions at the endothelial surface for tumor angiogenesis. Oncogene, 37, 4287-4299.
  • Ota F., Hirayama T., *Kizuka Y., Yamaguchi Y., Fujinawa R., Nagata M., Ismanto HS., Yamasaki S., Lepenies B., Aretz J., Rademacher C., Seeberger PH., Angata T., Kitazume S., Yoshida K., Betsuyaku T., Kida K., Taniguchi N. (2018) High affinity sugar ligands of C-type lectin receptor langerin. BBA Gen. Subj., 1862, 1592-1601. *corresponding author
  • Nakajima K., Kizuka Y., Yamaguchi Y., Hirabayashi Y., Takahashi K., Yuzawa Y., Taniguchi N. (2018) Identification and characterization of UDP-mannose in human cell lines and mouse organs: differential distribution across brain regions and organs. Biochem. Biophys. Res. Commun., 495, 401-407.


  • †Ota F., †*Kizuka Y., Nakano M., Yamaguchi Y., Kitazume S., Ookawara T., Taniguchi N. (2017) Sialylation of extracellular superoxide dismutase (EC-SOD) enhances furin-mediated cleavage and secretion. Glycobiology, 27, 1081-1088. †equal contribution, *corresponding author
  • Kizuka Y., Nakano M., Yamaguchi Y., Nakajima K., Oka R., Sato K., Hsu TL., Wong CH. Taniguchi N. (2017) An Alkynyl-fucose Halts Hepatoma Cell Migration and Invasion by Inhibiting GDP-Fucose Synthesizing Enzyme FX (TSTA3) Cell Chem. Biol., 24, 1467-1478.
  • Iijima J., Kobayashi S., Kitazume S., Kizuka Y., Fujinawa R., Korekane H., Shibata T., Saitoh S., Akashi-Takamura S., Miyake K., Miyoshi E., Taniguchi N. (2017) Core fucose is critical for CD14-dependent Toll-like receptor 4 signaling. Glycobiology, 27, 1006-1015.
  • Taichi M., Nomura S., Nakase I., Imamaki R., Kizuka Y., Ota F., Dohmae N., Kitazume S., Taniguchi N., Tanaka K. (2017) In-situ ligation of high- and low-affinity ligands to cell surface receptors enables highly selective recognition. Adv. Sci. 4, 1700147.
  • Sakuda K., Kizuka Y., Yamaguchi Y., Tanaka K., Ogiwara K., Segawa T., Hagiwara Y., Matsuo I., Ogawa H., Taniguchi N., Kitazume S. (2017) Reactivity of anti-HNK-1 antibodies to branched O-mannose glycans associated with demyelination. Biochem. Biophys. Res. Commun. 487, 450-456.
  • Kaburagi T., Kizuka Y., Kitazume S., Taniguchi N. (2017) Inhibitory Role of α2,6-Sialylation in Adipogenesis. J. Biol. Chem., 292, 2278-2286.
  • Gao C., Fujinawa R., Yoshida T., Ueno M, Ota F, Kizuka Y., Hirayama T., Korekane H., Kitazume S., Maeno T., Ohtsubo K., Yoshida K., Yamaguchi Y., Lepenies B., Aretz J., Rademacher C., Kabata H., Hegab AE., Seeberger PH., Betsuyaku T., Kida K., Taniguchi N. (2017) A keratan sulfate disaccharide prevents inflammation and the progression of emphysema in murine models. Am. J. Physiol. Lung Cell. Mol. Physiol., 312, L268-L276.


  • Ota F., Kizuka Y., Kitazume S., Adachi T., Taniguchi N. (2016) N-Glycosylation is essential for secretion of extracellular superoxide dismutase. FEBS Letters, 590, 3357-3367.
  • Kizuka Y., Funayama S., Shogomori H., Nakano M., Nakajima K., Oka R., Kitazume S., Yamaguchi Y., Sano M., Korekane H., Hsu TL., Lee HY., Wong CH., Taniguchi N. (2016). High sensitivity and low toxicity fucose probe for glycan imaging and biomarker discovery. Cell Chem. Biol., 23, 782-792.
  • Kizuka Y., Nakano M., Miura Y., Taniguchi N. (2016) Epigenetic regulation of neural N-glycomics. Proteomics, 16, 2854-2863.
  • Nagae M., Kanagawa M., Morita-Matsumoto K., Hanashima S., Kizuka Y., Taniguchi N., Yamaguchi Y. (2016) Atomic visualization of a flipped-back conformation of bisected glycans bound to specific lectins. Sci. Rep., 6, 22973.
  • Ogura A., Tahara T., Nozaki S., Morimoto K., Kizuka Y., Kitazume S., Hara M., Kojima S., Onoe H., Kurbangalieva A., Taniguchi N., Watanabe Y., Tanaka K. (2016) Visualizing Trimming Dependence of Biodistribution and Kinetics with Homo- and Heterogeneous N-Glycoclusters on Fluorescent Albumin. Sci. Rep., 6, 21797.
  • Kizuka Y., Nakano M., Kitazume S., Saito T., Saido T. C., Taniguchi N. (2016) Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J., 473, 21-30.


  • Yabuno K., Morise J., Kizuka Y., Hashii N., Kawasaki N., Takahashi S., Miyata S., Izumikawa T., Kitagawa H., Takematsu H., Oka S. (2015) A sulfated glycosaminoglycan linkage region is a novel type of Human Natural Killer-1 (HNK-1) epitope expressed on aggrecan in perineuronal nets. PLos One, 10, e0144560.
  • Ito E., Oka R., Ishii T., Korekane H., Kurimoto A., Kizuka Y., Kitazume S., Ariki S., Takahashi M., Kuroki Y., Kida K., Taniguchi N. (2015) Fucosylated surfactant protein-D is a biomarker candidate for the development of chronic obstructive pulmonary disease. J. Proteomics, 127, 386-394.
  • Kizuka Y., Kitazume S., Sato K., Taniguchi N. (2015) Clec4g (LSECtin) interacts with BACE1 and suppresses Ab generation. FEBS Letters, 589, 1418-1422.
  • Kizuka Y., Kitazume S., Fujinawa R., Saito T., Iwata N., Saido T.C., Nakano M., Yamaguchi Y., Hashimoto Y., Staufenbiel M., Hatsuta H., Murayama S., Manya H., Endo T., Taniguchi N. (2015) An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer’s disease. EMBO Mol. Med., 7, 175-189.


  • Okahara K., Kizuka Y., Kitazume S., Ota F., Nakajima K., Hirabayashi Y., Maekawa M., Yoshikawa T., Taniguchi N. (2014) Ceramide galactosyltransferase expression is regulated positively by Nkx2.2 and negatively by OLIG2. Glycobiology, 24, 926-934.
  • Akasaka-Manya K., Manya H., Kizuka Y., Oka S., Endo T. (2014) a-Klotho mice demonstrate increased expression of the non-sulfated N-glycan form of the HNK-1 glyco-epitope in kidney tissue. J. Biochem., 156, 107-113.
  • Kizuka Y., Kitazume S., Okahara K., Villagra A., Sotomayor E.M., Taniguchi N. (2014) Epigenetic regulation of a brain-specific glycosyltransferase N-acetylglucosaminyltransferase-IX (GnT-IX) by specific chromatin modifiers. J. Biol. Chem., 289, 11253-11261.
  • Kurimoto A., Kitazume S., Kizuka Y., Nakajima K., Oka R., Fujinawa R., Korekane H., Yamaguchi Y., Wada Y., Taniguchi N. (2014) The absence of core fucose up-regulates GnT-III and Wnt target genes: a possible mechanism for an adaptive response in terms of glycan function. J. Biol. Chem., 289, 11704-11714.
  • Morise J., Kizuka Y., Yabuno K., Tonoyama Y., Hashii N., Kawasaki N., Manya H., Miyagoe-Suzuki Y., Takeda S., Endo T., Maeda N., Takematsu H., Oka S. (2014) Structural and biochemical characterization of O-mannose-linked human natural killer-1 glycan expressed on phosphacan in developing mouse brains. Glycobiology, 24, 314-324.


  • Kanekiyo K., Inamori K., Kitazume S., Sato K., Maeda J., Higuchi M., Kizuka Y., Korekane H., Matsuo I., Honke K., and Taniguchi N. (2013) Loss of branched O-mannosyl glycans in astrocytes accelerates remyelination. J. Neurosci., 33, 10037-10047.
  • Kouno T., Kizuka Y., Nakagawa N., Yoshihara T., Asano M., Oka S. (2011) Specific enzyme complex of beta-1,4-galactosyltransferase-II and glucuronyltransferase-P facilitates biosynthesis of N-linked human natural killer-1 (HNK-1) carbohydrate. J. Biol. Chem., 286, 31337-31346.
  • Kizuka Y., Kitazume S., Yoshida M., Taniguchi N. (2011) Brain-specific expression of N-acetylglucosaminyltransferase IX (GnT-IX) is regulated by epigenetic histone modifications. J. Biol. Chem., 286, 31875-31884.
  • Kizuka Y., Tonoyama Y., and Oka S. (2009) Distinct transport and intracellular activities of two GlcAT-P isoforms. J. Biol. Chem., 284, 9247-9256.
  • Morita I., Kakuda S., Takeuchi Y., Itoh S., Kawasaki N., Kizuka Y., Kawasaki T., Oka S. (2009) HNK-1 glyco-epitope regulates the stability of the glutamate receptor subunit GluR2 on the neuronal cell surface. J. Biol. Chem., 284, 30209-30217.
  • Yoshihara T., Sugihara K., Kizuka Y., Oka S., Asano M. (2009) Learning/memory impairment and reduced expression of the HNK-1 carbohydrate in beta4-galactosyltransferase-II-deficient mice. J. Biol. Chem., 284, 12550-12561.
  • Kizuka Y., Kobayashi K., Kakuda S., Nakajima Y., Itoh S., Kawasaki N., Oka S. (2008) Laminin-1 is a novel carrier glycoprotein for the nonsulfated HNK-1 epitope in mouse kidney. Glycobiology, 18, 331-338.
  • Inoue M., Kato K., Matsuhashi H., Kizuka Y., Kawasaki T., Oka S. (2007) Distributions of glucuronyltransferases, GlcAT-P and GlcAT-S, and their target substrate, the HNK-1 carbohydrate epitope in the adult mouse brain with or without a targeted deletion of the GlcAT-P gene. Brain Res., 1179, 1-15.
  • Kizuka Y., Matsui T., Takematsu H., Kozutsumi Y., Kawasaki T., Oka S. (2006) Physical and functional association of glucuronyltransferases and sulfotransferase involved in HNK-1. J. Biol. Chem., 281, 13644-13651.
  • Tagawa H., Kizuka Y., Ikeda T., Itoh S., Kawasaki N., Kurihara H., Onozato ML, Tojo A., Sakai T., Kawasaki T., Oka S. (2005) A non-sulfated form of the HNK-1 carbohydrate is expressed in mouse kidney. J. Biol. Chem., 280, 23876-23883.

Review papers

  1. *Kizuka Y. (2022) Metabolic utilization and remodeling of glycan biosynthesis using fucose analogs. BBA Gen. Subj., 1866, 130243. *corresponding author.
  2. Harada Y., Ohkawa Y., Maeda K.,*Kizuka Y. ,Taniguchi N. (2021) Extracellular vesicles and glycosylation. Adv. Exp. Med. Biol., 1325, 137-149.
  3. Hirata T.,*Kizuka Y. (2021) N-Glycosylation. Adv. Exp. Med. Biol., 1325, 3-24. *corresponding author
  4. Hashimoto M., Fukamizu A., Nakagawa T.,*Kizuka Y. (2020) Roles of protein arginine methyltransferase 1 (PRMT1) in brain development and disease. BBA Gen. Subj., 1865, 129776.*corresponding author
  5. Taniguchi N., Ohkawa Y., Maeda K., Harada Y., Nagae M., Kizuka Y., Ihara H., Ikeda Y. (2020) True significance of N-acetylglucosaminyltransferases GnT-III, V and α1,6 fucosyltransferase in epithelial-mesenchymal transition and cancer. Mol. Aspects Med., in press.
  6. Nagae M., Yamaguchi Y., Taniguchi N., *Kizuka Y. (2020) 3D structure and function of glycosyltransferases involved in N-glycan maturation. Int. J. Mol. Sci., 21, 437. *corresponding author
  7. Harada Y., Ohkawa Y., Kizuka Y., Taniguchi N. (2019) Oligosaccharyltransferase: A gatekeeper of health and tumor progression. Int. J. Mol. Sci., 20, E6074.
  8. *Kizuka Y. (2019) Regulated expression and disease relevance of neural glycans. Trends Glycosci. Glycotech., 31, SE89-SE90. *corresponding author
  9. *Kizuka Y. (2018) Detection and modulation of fucosylated glycans using fucose analogs. Trends Glycosci. Glycotech., 31, E1-E32., *corresponding author
  10. *Kizuka Y., Mishra S., Yamaguchi Y., Taniguchi N. (2018) Implication of C-type lectin receptor langerin and keratan sulfate disaccharide in emphysema. Cell. Immunol., 333, 80-84. *corresponding author
  11. *Kizuka Y., Taniguchi N. (2018) Neural functions of bisecting GlcNAc. Glycoconj. J., 35, 345-351. *corresponding author
  12. *Kizuka Y., Kitazume S., Taniguchi N. (2017) N-glycan and Alzheimer’s disease. BBA Gen. Subj., 1861, 2447-2454. *corresponding author
  13. *Kizuka Y. (2017) Expression of neural glycans and their role in disease. Trends Glycosci. Glycotech., 29, E11-E18. *corresponding author
  14. Taniguchi N., Takahashi M., Kizuka Y., Kitazume S., Ookawara T., Furuta A. (2016) Glycation vs. Glycosylation: a tale of two different chemistries and biology in Alzheimer’s disease. Glycoconj. J., 33, 487-497.
  15. Takahashi M., Kizuka Y., Ohtsubo K., Gu J., Taniguchi N. (2016) Disease-associated glycans on cell surface proteins. Mol. Aspects of Med., 51, 56-70.
  16. Kizuka Y., Taniguchi N. (2016) Enzymes for N-Glycan Branching and Their Genetic and Nongenetic Regulation in Cancer. Biomolecules, 6, E25
  17. Taniguchi N., Kizuka Y., Takamatsu S., Miyoshi E., Gao CX., Suzuki K., Kitazume S., Ohtsubo K. (2015) Glyco-redox, a link between oxidative stress and changes of glycans: Lessons from research on glutathione, reactive oxygen and nitrogen species to glycobiology. Arch. Biochem. Biophys., 595, 72-80.
  18. Taniguchi N., Kizuka Y. (2015) Glycans and Cancer: Role of N-glycans in cancer biomarker, progression and metastasis and therapeutics. Adv. Cancer. Res., 126, 11-51.
  19. Kizuka Y., Kanekiyo K., Kitazume S., Taniguchi N. (2014) Use of Glycan-Targeted Antibodies/Lectins to Study the Expression/Function of Glycosyltransferases in the Nervous System. Adv. Neurobiol., 9, 117-127.
  20. Kizuka Y., and Oka S. (2012) Regulated expression and neural functions of human natural killer-1 (HNK-1) carbohydrate. Cell. Mol. Life Sci., 69, 4135-4147.
  21. Kizuka Y., Oka S. (2010) Regulation of HNK-1 (Human Natural Killer-1) carbohydrate expression: Multiple control mechanisms of biosynthetic enzyme activity. Trends Glycosci. Glycotech., 22, 194-199.
  22. Morita I., Kizuka Y., Kakuda S., Oka S. (2008) Expression and function of the HNK-1 carbohydrate. J. Biochem., 143, 719-724.