2018年以降の業績
Koizumi, K., Maeda, Y., Kano, T., Yoshida, H., Sakamoto, T., Yamagishi,
K. and Ueno, Y.*: Synthesis of 4'-C-aminoalkyl-2'-O-methyl modified RNA
and their biological properties. Bioorg. Med. Chem. 26: 521-3534, 2018.
Nakamoto, K., Akao, Y. and Ueno, Y.*: Diazirine-containing tag-free RNA
probes for efficient RISC-loading and photoaffinity labeling of microRNA
targets. Bioorg. Med. Chem. Lett. 28: 2906-2909, 2018.
Nakamoto, K., Akao, Y., Furuichi, Y. and Ueno, Y.*: Enhanced intercellular
delivery of cRGD-siRNA conjugates by an additional oligospermine modification.
ACS Omega. 3: 8226-8232, 2018.
Kano, T., Katsuragi, Y., Maeda, Y. and Ueno, Y.*: Synthesis and properties
of 4'-C-aminoalkyl-2'-fluoro-modified RNA oligomers. Bioorg. Med. Chem.
26: 4574-4582, 2018.
Chandela, A. and Ueno, Y.*: Systemic delivery of small interfering RNA
therapeutics; obstacles and advances. Reviews in Agricultural Science 7:
10-28, 2019.
Chandela, A., Watanabe, T., Yamagishi, K. and Ueno Y.*: Synthesis and characterization
of small interfering RNAs with haloalkyl groups at their 3'-dangling ends.
Bioorg. Med. Chem. 27:1341-1349, 2019.
Kajino, R., Maeda, Y., Yoshida, H., Yamagishi, K. and Ueno, Y.*: Synthesis
and biophysical characterization of RNAs containing (R)- and (S)-5'-C-aminopropyl-2'-O-methyluridines.
J. Org. Chem. 84: 3388-3404, 2019.
Chandela, A. and Ueno, Y.*: Design, synthesis and evaluation of novel,
branched trident small interfering RNA nanostructures for sequence-specific
RNAi activity. RSC Advances 9: 34166-34171, 2019.
Uematsu, A., Kajino, R., Maeda, Y. and Ueno, Y.*: Synthesis and characterization
of 4'-C-guanidinomethyl-2'-O-methyl-modified RNA oligomers. Nucleotides
& Nucleic Acids 39: 280-291, 2020.
Yoshikawa, R., Heishima, K., Ueno, Y., Kawade, M., Maeda, Y., Yoshida,
K., Murakami, M., Sakai, H., Akao, Y. and Mori, T.: Development of synthetic
microRNA-214 showing enhanced cytotoxicity and RNase resistance for treatment
of canine hemangiosarcoma. Veterinary and Comparative Oncology 18: 570-579,
2020.
Tsuchihira, T., Kajino, R., Maeda, Y. and Ueno, Y.*: 4'-C-Aminomethyl-2'-deoxy-2'-fluoroarabinonucleoside
increases the nuclease resistance of DNA without inhibiting the ability
of a DNA/RNA duplex to activate RNase H. Bioorg. Med. Chem. 28: 115611,
2020.
梶野瞭平，上野義仁*: アンチセンス核酸の毒性発現メカニズムと毒性発現低減に向けた科学的アプローチ. 日本核酸医薬学会誌, 35-43, 2020.
Matsubara, M., Honda, K., Ozaki, K., Kajino, R., Kakisawa, Y., Maeda, Y.
and Ueno, Y.*: Synthesis of siRNAs incorporated with cationic peptides
R8G7 and R8A7 and the effect of the modifications on siRNA properties.
RSC Advances 10: 34815-34824, 2020.
Takeuchi, A., Hisamatsu, K., Okumura, N., Sugimitsu, Y., Yanase, E., Ueno,
Y. and Nagaoka, S.: IIAEK targets intestinal alkaline phosphatase (IAP)
to improve cholesterol metabolism with a specific activation of iap and
downregulation of ABCA1. Nutrients 12: 2859, 2020.
Zhou, Y., Kajino, R., Ishii, S., Yamagishi, K. and Ueno, Y.*: Synthesis
and evaluation of (S)-5'-C-aminopropyl and (S)-5'-C-aminopropyl-2'-arabinofluoro
modified DNA oligomers for novel RNase H-dependent antisense oligonucleotides.
RSC Advances 10: 41901-41914, 2020.
上野義仁*，梶野瞭平: アンチセンス核酸の合成. In: 核酸科学ハンドブック．杉本直己 編．講談社，pp. 513-515, 2020．
Kajino, R. and Ueno, Y.*: (S)-5'-C-Aminopropyl-2'-O-methyl nucleosides
enhance antisense activity in cultured cells and binding affinity to complementary
single-stranded RNA. Bioorg. Med. Chem. 30: 115925, 2021.
Tsukimura, R., Kajino, R., Zhou, Y., Chandela, A. and Ueno, Y.*: 4'-C-Aminoethoxy
modification enhanced nuclease resistance of RNAs and improved thermal
stability of RNA duplexes. Results in Chemistry 3: 100231, 2021.
梶野 瞭平,上野義仁*: 核酸医薬を指向したカチオン性側鎖を有する新規人工核酸の合成, 有機合成化学協会誌, 79: 1102-1112, 2021.
Yoshikawa, R., Maeda, A., Ueno, Y., Sakai, H., Kimura, S., Sawadaishi,
T., Kohgo, S., Yamada, K. and Mori, T.: Intraperitoneal administration
of synthetic microRNA-214 elicits tumor suppression in an intraperitoneal
dissemination mouse model of canine hemangiosarcoma. Veterinary Research
Communications, 2022. doi.org/10.1007/s11259-021-09869-1
Ueda, H. and Ueno, Y.*: Synthesis of 4'-C-(aminoethyl)thymidine and 4'-C-[(N-methyl)aminoethyl]thymidine
by a new synthetic route and evaluation of the properties of the DNAs containing
the nucleoside analogs. Bioorg. Med. Chem. 60: 116690, 2022.
Kajino, R., Sakamoto, S. and Ueno, Y.*: Synthesis, gene silencing activity,
thermal stability, and serum stability of siRNA containing four (S)-5'-C-aminopropyl-2'-O-methylnucleosides
(A, adenosine; U, uridine; G, guanosine; and C, cytidine). ASC Advances
12: 11454–11476, 2022.
Katsuzaki, Y., Tsukimura, R., Chandela, A., Chano, T. and Ueno, Y.*: 4'-C-Aminoethoxy-modified
DNAs exhibit increased nuclease resistance, sustained RNase H activity,
and inhibition of KRAS gene expression. Chem. Biodiversity 19: e202200125,
2022.
Chandela, A., Ueda, H. and Ueno, Y.*: Synthesis of 4'-C-(Aminoethyl)thymidine
and 4'-C-[(N-methyl)aminoethyl]thymidine nucleosides to enhance DNA stability.
Current Protocols 2(9): e501, 2022.
Zhou, Y., Sakamoto, S. and Ueno, Y.*: Antisense gapmers with LNA-Wings
and (S)-5'-C-Aminopropyl-2'-arabinofluoro-nucleosides could efficiently
suppress the expression of KNTC2. Molecules 27(21): 7384, 2022.
Sato, H.，Chandela，A., Ueno, Y.*: Synthesis and characterization of novel
(S)-5'-C-aminopropyl-2'-fluorouridine modified oligonucleotides as therapeutic
siRNAs，Bioorg. Med. Chem. 87 (2023) 117317，https://doi.org/10.1016/j.bmc.2023.117317
Koyasu, K., Chandela，A., Ueno, Y.*: Non-terminal conjugation of small interfering
RNAs with spermine improves duplex binding and serum stability with position-specific
incorporation, RSC Advances, 13, 25169–25181, 2023. https://doi.org/10.1039/D3RA04918C
Sawada, H., Kakisawa, Y. Ueno, Y.*: Properties and synergistic effects
of a nonionic backbone and aminoalkyl modified nucleosides in RNAs, Bioorganic
Chemistry, 144:107143, 2024. DOI:10.1016/j.bioorg.2024.107143
梶野瞭平, 佐藤仁昴, 上野義仁*: 糖部5′-位アミノアルキル修飾ヌクレオシドの合成とsiRNA医薬への応用. 核酸医薬, 第2章 第2節,
75-82, 2024.
Onishi, S., Chandela, A. Ueno, Y.*: Synthesis and Evaluation of 4′-C-(S)
and 4′-C-(R)-2-aminopropoxy-thymidine-modified DNAs for Thermal Stability,
RNase H Digestion, and Nuclease Resistance, ChemistrySelect 2024, 9, e2023050.
https://doi.org/10.1002/slct.202305090
Hibino, H., Zhou, Y., Saito, Y., Ueno, Y.*: Novel synthesis and evaluation
of oligonucleotides containing (S)-5′-C-aminopropyl-modified thymidine
analogs for RNase H-dependent antisense therapy, Results in Chemistry,
9 (2024) 101648. https://doi.org/10.1016/j.rechem.2024.101648
Zhou, Y., Sato, H., Kawade, M., Yamagishi, K., Ueno, Y.*: Application of 4′-C-α-aminoethoxy-2′-O-methyl-5-propynyl-uridine for antisense therapeutics, RSC Advances, 2024, 14, 39148–39162. https://doi.org/10.1039/d4ra06376g
Fujiki, K., Kakisawa, Y., Mahmoud, M. E., Ueno, Y.*: Synthesis and application of 4′-C-[(N-alkyl)aminoethyl]thymidine analogs for optimizing oligonucleotide properties, Molecules 2025, 30, 581. https://doi.org/10.3390/molecules30030581
Hayashi, H., Seki, S., Tomita, T., Kato, M., Ashihara, N., Chano, T., Sanjo, H., Kawade, M., Yan, C., Sakai, H., Tomida, H., Tanaka, M., Iwaya, M., Taki, S., Nakazawa, Y., Soejima, Y., Ueno, Y., Hiratsuka, S.: Synthetic short mRNA prevents metastasis via innate-adaptive immunity, Nature Communications, (2025) 16:1925. DOI: 10.1038/s41467-025-57123-y