research area

 



  1. 2.Analysis of Transcriptional Network at the Heart of Environmental Adaptation of Higher Plants


   Sessile plants have evolved excellent ability to adapt to a wide range of environmental conditions for their survival. The heart of the ability is the transcriptional response to environmental change that is implemented by the transcriptional network of higher plants. Currently, most of the network is hidden in a veil of mystery. We are trying to reveal this network through several approaches, including bioinformatics, functional genomics, and biochemistry.



keywords:

   environmental response, large-scale expression

   analysis, NGS, CAGE, RNAseq, promoter prediction, AlphaScreen,  transcriptional network, signal crosstalk, TF relay, cis-trans pairing, systems biology.







  1. 3.Transcriptional Response to Light Stress


   Light is necessary for plants to grow as the energy source of photosynthesis, but excess light damages photosynthetic apparatus, chloroplasts, and tissues, inhibits plant growth and threatens plant’s life. In addition, light stress can be observed at other stress conditions, such as cold, UV-B, drought, and nutrition-deficient stresses. We are trying to understand these stresses in a view of light stress. Methods of population genetics (GWAS) are also utilized.



keywords:

   light stress, cold stress, UV-B stress, photoinhibition,

   chlorophyll fluorescence, PAM, photosynthetic

   electron transfer, state transition, ROS, redox

   regulation, photoprotection, ELIP, phenylpropanoid

   biosynthesis,  




  1. 4.Promoter Structure and Evolution


   Plant Promoters are not static in the nucleotide sequence. Thanking to the great power of NGS, evolution of plant promoters are studied in order to understand genetic adaptation of plants to environments. Methods of population genetics (GWAS) are also utilized.


keywords:

   regulatory SNP, transposon, GWAS, local adaptation, ecogenomics.




  1. 5.Photosynthetic Seaslug


   Some seaslugs in sacoglossa are able to photosynthesize by a phenomenon called “kleptoplasty”.


keywords:

   secondary endosymbiosis, kleptoplasty, seaslug, green algae.

  

























































keywoords

 
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OUR RESEARCH    


  1. 1.Genome-Wide Promoter Analysis


   In our lab, computational and experimental analyses are combined to elucidate plant promoter structure. Synthetic promoters are also utilized to validate predicted cis-regulatory elements and to understand the transcriptional network in higher plants.  Some of the results can be accessed at our plant promoter database (ppdb, http://ppdb.agr.gifu-u.ac.jp).



keywords:

   TSS, NGS, CapTrapper, CAGE, LDSS, in silico analysis,

   promoter structure, core promoter, transcriptional

   regulatory element.





topics:

   Brief Introduction of LDSS  

   ppdb: Plant Promoter Database

   Guide to Reporter Genes

   Heterogeneity of Plant Core Promoter

 

Highly Cited Researchers of Gifu University

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