Real-time PCR protocols
These are the protocols used in our technical training course.
You can download them from following links (PDF files).
1 Real-time quantitative PCR schedule (約610kb)
2 Reverse Transcription (約459kb)
3 Test experiment1
Check amplification and Ct value (1st PCR) (約557kb)
4 Test experiment2
Determination of the range of calibration curve (2nd PCR) (約768kb)
5 Real-time quantitative PCR experiment (Comparison of gene expression) (約767kb)
Primer design (約1.68mb)
Supplemental1 table (約30.5kb)
Supplemental2 table (約30.5kb)
Ct value interpretation1 (約589kb)
Ct value interpretation2 (約589kb)