Dr. Ryo Ishiguro
■Assistant Professor
Biomolecular Science Course
Department of Chemistry and Biomolecular Science
Gifu University
Degree
Ph. D. in Science
Research
Characterizarion of protein association system using high-pressure native PAGE
Expansion of native PAGE into the quantitative probe for the protein association system
Native polyacrylamide gel electrophoresis (PAGE) is a conventional technique to separate and characterize the protein complexes in their native and active forms. Various analysis methods have been established to determine the particle size in PAGE, containing gradient-gel electrophoresis, Ferguson plot analysis, two-dimensional electrophoresis, etc. On the other hand, two significant drawbacks remain to be eliminated upon its application to the physicochemical study of the protein association; 1) modifying the association equilibrium in situ, and 2) quantifying the each band, are difficult.
We aim to expand native PAGE into the probe applicable to the study on the protein association equilibrium. Our originally developed high-pressure electrophoresis apparatus enables to perturb the association equilibrium, and the newly emerged band of association intermediates or monomeric components could be characterized using PAGE analysis. Additionally these bands could be quantified by image analysis of stained gels, leading to the thermodynamic analysis of the temperature- and pressure dependent of the association equilibrium.
Classes
Undergraduate
None
Graduate
None
