岐阜大学大学院医学系研究科組織・器官形成分野

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1. Miyamoto, A., Kunisada, T., Hemmi, H., Yamane, T., Yasuda, H., Miyake, K., Yamazaki, H., Hayashi, S-I. Establishment and characterization of an immortal macrophage-like cell line inducible to differentiate to osteoclasts. Biochem. Biophys. Res. Commun. 242, 703-709, 1998.

2. Kunisada, T., Lu, S-Z., Yoshida, H., Nishikawa, S., Nishikawa, S-I., Mizoguchi, M., Hayashi, S-I., Tyrrell, L., Williams, D.A., Longley, B.J. Murine cutaneous mastocytosis and epidermal melanocytosis induced by keratinocyte expression of transgenic stem cell factor. J. Exp. Med. 187, 1565-1573, 1998.

3. Ogawa, K., Wakayama, A., Kunisada, T., Orii, H., Watanabe, K., Agata, K. Identification of a receptor tyrosine kinase involved in germ cell differentiation in planarians. Biochem. Biophys. Res. Commun. 248, 204-209, 1998.

4. Kunisada, T., Yoshida, H., Yamazaki, H. Miyamoto, A., Hemmi, H., Nishimura, E., Shultz, L.D., Nishikawa, S-I., Hayashi, S-I. Transgene expression of steel factor in the basal layer of epidermis promotes survival, proliferation, differentiation and migration of melanocyte precursors. Development 125, 2915-2923, 1998.

5. Miyamoto, A., Kunisada, T., Yamazaki, H., Miyake, K., Nishikawa, S-I., Sudo, T., Shultz, L.D., Hayashi, S-I. Establishment and characterization of pro-B cell lines from motheaten mutant mouse defective in SHP-1 protein tyrosine phosphatase. Immunol. Letters 63, 75-82, 1998.

6. Yamazaki, H., Kunisada, T., Ishizu, A., Ikeda, H., Miyoshi, I., Sudo, T., Hayashi, S.I., Yoshiki, K. Promotion of early osteoclastogenesis and B lymphopoiesis in the bone marrow of transgenic rats with the env-pX gene of human T-cell lymphotropic virus type I. Oncogene 17, 2955-2960, 1998.

7. Ogawa, M., Sugawara, S., Kunisada, T., Sudo, T., Hayashi, S-I., Nishikawa, S., Kodama, H., Nishikawa, S-I. Flt3/Flk2 and c-Kit are not essential for the proliferation of B lymphoid progenitor cells in the bone marrow of the adult mouse. Exp. Hematol., 26, 478-488, 1998.

8. Tanio, Y., Yamazaki, H., Kunisada, T., Miyake, K., Hayashi, S-I. CD9 molecule expressed on stromal cells is involved in osteoclastogenesis. Exp. Hematol. 27, 853-859, 1999.

9. Yamazaki, H., Kunisada, T., Miyamoto, A., Tagaya, H., Hayashi, S-I. Tooth-specific expression conferred by the regulatory sequences of rat dentin sialoprotein 2 gene in transgenic mice. Biochem. Biophys. Res. Commun. 260, 433-440, 1999.

10. Nishimura, E.K., Yoshida, H., Kunisada, T., Nishikawa, S-I. Cadherin expression associated with melanocyte migration and diversification reflects the microenvironmental setting. Dev. Biol. 215, 155-166, 1999.

11. Yamane, T., Hayashi, S.I., Mizoguchi, M., Yamazaki, H., Kunisada, T. Derivation of melanocytes from embryonic stem cells in culture. Dev. Dyn. 216, 450-458, 1999.

12. Shiota, G., Kunisada, T., Oyama, K., Udagawa, A., Nomi, T., Tanaka, K., Tsutsumi, A., Isono, M., Nakamura, T., Hamada, H., Sakatani, T., Sell, S., Ito, H., Kawasaki, H. In vivo transfer of hepatocyte growth factor gene accelerates proliferation of hepatic oval cells in a 2-acetylaminofluorene/partial hepatectomy model in rats. FEBS Letters. 470, 325-330, 2000.

13. Kunisada, T., Yamazaki, H., Hirobe, T., Kamei, S., Omoteno, M., Tagaya, H., Hemmi, H., Koshimizu, U., Nakamura, T., Hayashi, S-I. Keratinocyte expression of transgenic hepatocyte growth factor (HGF) affects melanocyte development, leading to dermal melanocytosis. Mech. Dev. 94, 67-78, 2000.

14. Tagaya, H., Kunisada, T., Yamazaki, H., Yamane, T., Tokuhisa, T., Wagner, E., Sudo, T., Shultz, L.D., Hayashi, S-I.  Intramedullary and extramedullary B lymphopoiesis in osteopetritic mice.  Blood 95, 3363-3370, 2000.

15. Kamei, S., Yajima, I., Yamamoto, H., Kobayashi, A., Makabe, K.W., Yamazaki, H., Hayashi, S-I., Kunisada, T. Characterization of a novel member of the FGFR family, HrFGFR, in Halocynthia roretzi. Biochem Biophys Res Commun. 275, 503-508, 2000.

16. Yamane, T., Kunisada, T., Yamazaki, H., Nakano, T., Orkin, S.H., Hayashi, S-I. Sequential requirements for SCL/tal-1, GATA-2, M-CSF and ODF/OPGL ligand in osteoclast  development.  Exp. Hematol. 28, 833-840, 2000.

17. Hemmi, H., Okuyama, H., Yamane, T., Nishikawa, S-I., Nakano, T., Yamazaki, H., Kunisada, T.  and Hayashi S-I. Temporal and spatial localization of osteoclasts in colonies from embryonic stem cells.  Biochem. Biophys. Res. Commun. 280, 526-534, 2001.

18. Hemmi, H., Yoshino, M., Yamazaki, H., Naito, M., Iyoda, T., Omatsu, Y., Shimoyama, S., Letterio, J.J., Nakabayashi, T., Tagaya, H., Yamane, T., Ogawa, M., Nishikawa, S.I., Ryoke, K., Inaba, K., Hayashi, S.I., Kunisada, T. Skin antigens in the steady state are trafficked to regional lymph nodes by transforming growth factor-b1-dependent cells.  Int. Immunol. 13, 695-704, 2001.

19. Wang, K., Gan, L., Kunisada, T., Lee, I., Yamagishi, H. and Hood, L. Characterization of the Japanese pufferfish (Takifugu rubripes) T-cell receptor alpha locus reveals a unique genomic organization.  Immunogenet. 53, 31-42, 2001.

20. Yamane, T., Kunisada, T., Tsukamoto, H., Yamazaki, H., Niwa, H., Takada, S. Hayashi, S-I. Wnt signaling regulates hemopoiesis through stromal cells. J. Immunol. 167, 765-772, 2001.

21. Kunisada, T., Yamazaki, H., Hayashi, S.I. Ligands for receptor tyrosine kinases expressed in the skin as environmental factors for melanocyte development. J. Invest. Dermatol. 6, 6-9, 2001.

22. Yoshida, H., Kunisada, T., Grimm, T., Nishimura, E., Nishioka, E., Nishikawa, S-I. Melanocyte migration and survival controlled by SCF/c-kit expression. J. Invest. Dermatol. 6, 1-5, 2001.

23. Yamane, T. Hayashi, S.I., Kunisada, T. Embryonic stem cells as a model to study melanocyte lineage. Methods Mol. Biol. 185, 261-268, 2002.

24. Yamane, T., Kunisada, T., Hayashi, S.I. Embryonic stem cells as a model to study osteoclast lineage. Methods Mol. Biol. 185, 97-106, 2002.

25. Yoshino, M., Yamazaki, H., Yoshida, H., Niida, S., Nishikawa, S., Ryoke, K., Kunisada, T., Hayashi, S.I. Reduction of osteoclasts in a critical embryonic period is essential for inhibition of mouse tooth eruption.  Bone Miner Res. 18, 108-116, 2003.

26. Kunisada, T., Yamane, T., Aoki, H., Yoshimura, N., Ishizaki, K., Motohashi, T. Development of melanocytes from ES cells. Methods Enzymol. 365, 341-349, 2003.

27. Kokuzawa, J., Yoshimura, S., Kitajima, H., Shinoda, J., Kaku, Y., Iwama, T., Morishita, R., Shimazaki, T., Okano, H., Kunisada, T., Sakai, N. Hepatocyte growth factor promotes proliferation and neuronal differentiation of neural stem cells from mouse embryos. Mol. Cell Neurosci. 24, 190-197, 2003.

28. Hirano, M., Yamamoto, A., Yoshimura, N., Tokunaga, T., Motohashi, T., Ishizaki, K., Yoshida, H., Okazaki, K.,Yamazaki, H., Hayashi, S.I., Kunisada, T. Generation of structures formed by lens and retinal cells differentiating from embryonic stem cells.  Dev. Dyn. 228, 664-671, 2003.

29. Hara, A., Niwa, M., Kunisada, T.,Yoshimura, N., Katayama, M., Kozawa, O., Mori, H.  Embryonic stem cells are capable of generating a neuronal network in the adult mouse retina.  Brain Res. 999, 216-221, 2004.

30. Pla P, Solovieva O, Moore R, Alberti C, Kunisada T, Larue L. Dct::lacZ ES Cells:Novel Cellular Model to Study Melanocyte Determination and Differentiation.  Pigment Cell Res. 17, 142-149, 2004.

31. Kato M, Yoshimura S, Kokuzawa J, kitajima H, Kaku Y, Iwama T, Shinoda J, Kunisada T, Sakai N.  Hepatocyte growth factor promotes neuronal differentiation of neural stem cells derived from embryonic stem cells.  Neuro Report 15, 5-8, 2004.

32. Kato M, Takeda K, Kawamoto Y, Tsuzuki T, Hossain K, Tamakoshi A, Kunisada T, Kambayashi Y, Ogino K, Suzuki H, Takahashi M, Nakashima I. c-Kit-targeting immunotherapy for hereditary melanoma in a mouse model. Cancer Res. 64, 801-806, 2004.

33. Yanagitani A, Yamada S, Yasui S, Shimomura T, Murai R, Murawaki Y, Hashiguchi K, Kanbe T, Saeki T, Ichiba M, Tanabe Y, Yoshida Y, Morino S, Kurimasa A, Usuda N, Yamazaki H, Kunisada T, Ito H, Murawaki Y, Shiota G. Retinoic acid receptor alpha dominant negative form causes steatohepatitis and liver tumors in transgenic mice. Hepatology. 40, 366-375, 2004.

34. Yamazaki F, Okamoto H, Miyauchi-Hashimoto H, Matsumura Y, Itoh T, Tanaka K, Kunisada T, Horio T. XPA gene-deficient, SCF-transgenic mice with epidermal melanin are resistant to UV-induced carcinogenesis.  J. Invest. Dermaol. 123220-2282004.

35. Kitajima H, Yoshimura S, Kokuzawa J, Kato, M, Iwama T, Motohashi T, Kunisada T, Sakai N.  A culture method for the induction of neuroshperes from mouse embryonic stem cells by co-culture with PA6 stromal cells.  Mol. Cell Neurosci. 80, 467-474, 2004.

36. Aoki H, Motohashi T, Yoshimura N, Yamazaki H, Yamane T, Panthier JJ, Kunisada T.  Cooperative and indispensable roles of endothelin 3 and KIT signalings in melanocyte development. Dev. Dyn. 233, 407-417, 2005.

37. Yamazaki H, Sakata E, Yamane T, Yanagisawa A, Abe K, Yamamura KI, Hayashi SI, Kunisada T. Presence and distribution of neural crest-derived cells in the murine developing thymus and their potential for differentiation. Int. Immunol. 17, 549-558, 2005.

38. Pla P, Alberti C, Solovieva O, Pasdar M, Kunisada T, Larue L.  Ednrb2 orients cell migration towards the dorsolateral neural crest pathway and promotes melanocyte differentiation. Pigment Cell Res. 18, 181-187, 2005.

39. Kurita K, Nishito M, Shimogaki H, Takada K, Yamazaki H, Kunisada T.  Suppression of progressive loss of coat color in microphthalmia-vitiligo mutant mice.  J. Invest. Dermatol. 125, 538-544, 2005.

40. Aoki H, Hara A, Nakagawa S, Motohashi T,  Hirano M, Takahashi Y, Kunisada T.  Embryonic stem cells that differentiate into RPE cell precursors in vitro develop into RPE cell monolayers in vivo. Exp. Eye Res. 82, 265-274, 2006.

41. Tosaki H, Kunisada T, Motohashi T, Aoki H, Yoshida H, Kitajima Y. Mice transgenic for Kit(V620A): recapitulation of piebaldism but not progressive depigmentation seen in humans with this mutation. J. Invest. Dermatol. 126, 1111-1118, 2006.

42. Takahashi T, Kawai T, Ushikoshi H, Nagano S, Oshika H, Inoue M, Kunisada T, Takemura G, Fujiwara H, Kosai KI. Identification and Isolation of Embryonic Stem Cell-Derived Target Cells by Adenoviral Conditional Targeting. Mol Ther. 14, 673-683, 2006.

43. Hara A, Niwa M, Kumada M, Aoki H, Kunisada T, Oyama T, Yamamoto T, Kozawa O, Mori H. Intraocular injection of folate antagonist methotrexate induces neuronal differentiation of embryonic stem cells transplanted in the adult mouse retina. Brain Res. 1085, 33-42, 2006.

44. Motohashi, T., Aoki, H., Yoshimura, N., Kunisada, T. Induction of melanocytes from embryonic stem cells and their therapeutic potential. Pigment Cell Res. 19, 284-289, 2006.

45. Hara A, Niwa M, Aoki H, Kumada M, Kunisada T, Oyama T, Yamamoto T, Kozawa O, Mori H. A new model of retinal photoreceptor cell degeneration induced by a chemical hypoxia-mimicking agent, cobalt chloride. Brain Res. 1109, 192-200, 2006.

46. D'Orazio JA, Nobuhisa T, Cui R, Arya M, Spry M, Wakamatsu K, Igras V, Kunisada T, Granter SR, Nishimura EK, Ito S, Fisher DE. Topical drug rescue strategy and skin protection based on the role of Mc1r in UV-induced tanning. Nature 443, 340-344, 2006.

47. Suetsugu A, Nagaki M, Aoki H, Motohashi T, Kunisada T, Moriwaki H. Characterization of CD133+ hepatocellular carcinoma cells as cancer stem/progenitor cells. Biochem Biophys Res Commun. 351, 820-824.2006.

48. Motohashi T, Aoki H, Chiba K, Yoshimura N, Kunisada T. Multipotent cell fate of neural crest-like cells derived from embryonic stem cells. Stem cells 25, 402-410, 2007.

49. Suetsugu A, Nagaki M, Aoki H, Motohashi T, Kunisada T, Moriwaki H. Characterization of CD133+ hepatocellular carcinoma cells as cancer stem/progenitor cells. Biochem Biophys Res Commun. 351, 820-824.2006.

50. Aoki H, Hara A, Niwa M, Motohashi T, Suzuki T, Kunisada T. An in vitro mouse model for retinal ganglion cell replacement therapy using eye-like structures differentiated from ES cells. Exp Eye Res. 84, 868-875, 2007.

51. Miwa M, Inoue-Murayama M, Aoki H, Kunisada T, Hiragaki T, Mizutani M, Ito S. Endothelin receptor B2 (EDNRB2) is associated with the panda plumage colour mutation in Japanese quail. Anim Genet. 38, 103-108, 2007.

52. Tomita H, Yamada Y, Oyama T, Hata K, Hirose Y, Hara A, Kunisada T, Sugiyama Y, Adachi Y, Linhart H, Mori H. Development of gastric tumors in Apc Min/+ mice by the activation of the β-catenin/Tcf signaling pathway. Cancer Res.  67, 4079-4087, 2007.

53. Hara A, Taguchi A, Niwa H, Aoki H, Yamada Y, Ito H, Nagata K,  Kunisada T, Mori H. Localization of septin 8 in murine retina, and spatiotemporal expression of septin 8 in a murine model of photoreceptor cell degeneration. Neuroscience Letters 423, 205-210, 2007.

54. Inagaki A,  Soeda A,  Oka N,  Kitajima H,  Nakagawa J, Motohashi T, Kunisada T, Iwama T. Long- term maintenance of brain tumor stem cell properties under at non-adherent and adherent culture conditions. Biochem Biophys Res Commun. 361, 586-592, 2007.

55. Oka N, Soeda A, Inagaki A, Onodera M, Maruyama H, Hara A, Kunisada T, Mori H, Iwama T. VEGF promotes tumorigenesis and angiogenesis of human glioblastoma stem cells. Biochem Biophys Res Commun. 360, 553-559, 2007.

56. Aoki H, Hara A, Niwa,M, Motohashi T, Siziki T, Kunisada T. Transplantation of cells from eye-like structures differentiated from embryonic stem cells in vitro and in vivo regeneration of retinal ganglion-like cells. Grafe’s Arc Clin Exp Optalmol. 246, 255-265, 2008.

57. Kawaguchi A, Chiba K, Tanimura Y, Motohashi T, Aoki H, Takeda T, Hayashi S, Shimizu K, Kunisada T. Isolation and characterization of Kit-independent melanocyte precursors induced in the skin of Steel factor transgenic mice. Dev Growth Differ. 50, 63-69, 2008.

58. Oyama T, Yamada Y, Hata K, Tomita H, Hirata A, Sheng H, Hara A, Aoki H, Kunisada T, Yamashita S, Mori H. Further upregulation of b-catenin/Tcf transcription is involved in the development of macroscopic tumors in the colon of Apc Min/+ mice. Carcinogenesis 29, 666-672, 2008.

59. Soeda A, Inagaki A, Oka N, Ikegame Y, Aoki H, Yoshimura SI, Nakashima S, Kunisada T, Iwama T. Epidermal growth factor plays a crucial role in mitogenic regulation of human brain tumor stem cells. J Biol Chem. 283, 586-592, 2008.

60. Aoki H, Yoshida H, Hara A, Suzuki T, Kunisada T. Transplantation of melanocytes into iris: method for iris repigmentation. Transplantation. 85, 492-494, 2008.

61. Hara A, Aoki H, Taguchi A, Niwa M, Yamada Y, Kunisada T, Mori H. Neuron-like Differentiation and Selective Ablation of Undifferentiated Embryonic Stem Cells Containing Suicide Gene with Oct-4 Promoter. Stem Cells Dev. 17, 619-627, 2008.

62. Aoki H, KunisadaT. Colourless side of the nude mutation: Foxn1 and hair pigmentation. Pigment Cell Melanoma Res. 21, 338-339, 2008

63. Takeda T, Tezuka Y, Horiuchi M, Hosono K, Iida K, Hatakeyama D, Miyaki S, Kunisada T, Shibata T, Tezuka K. Characterization of dental pulp stem cells of human tooth germs. J Dent Res. 87, 676-681, 2008.

64. Tsuji Y, Yoshimura N, Aoki H, Sharov AA, Ko MS, Motohashi T, Kunisada T. Maintenance of undifferentiated mouse embryonic stem cells in suspension by the serum- and feeder-free defined culture condition. Dev Dyn. 237, 2129-2138, 2008.

65. Aoki H, Hara A, Motohashi T, Chem H, Kunisada T. Iris as a recipient tissue for pigment cells: organized in vivo differentiation of melanocytes and pigmented epithelium derived from embryonic stem cells in vitro. Dev Dyn. 237, 2394-2404, 2008.

66. Hayashi H, Kunisada T, Takakura N, Aoki M, Mizuta K, Ito Y. Involvement of platelet-derived growth factor receptor-beta in maintenance of mesenchyme and sensory epithelium of the neonatal mouse inner ear. Hearing Res. 245, 73-81, 2008.

67. Shimada H, Yoshimura N, Tsuji A, Kunisada T. Differentiation of dopaminergic neurons from human embryonic stem cells: modulation of differentiation by FGF-20. J Biosci Bioeng. 107:447-454, 2009.

68. Motohashi T, Yamanaka K, Chiba K, Aoki H, Kunisada T. Unexpected multipotency of melanoblasts isolated from murine skin. Stem Cells 27, 888-897, 2009.

69. Aoki H, Yamada Y, Hara A, Kunisada T. Two distinct types of mouse melanocyte: differential signaling requirement for the maintenance of non-cutaneous and dermal versus epidermal melanocytes. Development 136, 2511-2521, 2009.

70. Aoki H, Hara A, Niwa M, Yamada Y, Kunisada T. In vitro and in vivo differentiation of human embryonic stem cells into retina-like organs and comparison with that from mouse pluripotent epiblast stem cells.Aoki H. Dev Dyn. 238, 2266-2279, 2009.

71. Soeda A, Park M, Lee D, Mintz A, Androutsellis-Theotokis A, McKay RD, Engh J, Iwama T, Kunisada T, Kassam AB, Pollack IF, Park DM. Hypoxia promotes expansion of the CD133-positive glioma stem cells through activation of HIF-1alpha. Oncogene 28, 3949-3959, 2009.

 

72. Yamada, Y, Aoki, H, Kunisada, T, Hara, A. Rest promotes the early differentiation of mouse ESCs but is not required for their maintenance. Cell Stem Cell in press.

 

 

國貞隆弘、マスト細胞症とSCF、臨床免疫、32, 706-711, 1999.

美藤荘二、作美礼乃、小林幹朗、山下慶三、橋本易周、林眞一、國貞隆弘、皮膚特異的にSCFを発現させたトランスジェニックマウス、アレルギー科、9, 267-273, 2000.

國貞隆弘、山根利之、林眞一、ES細胞からの色素細胞の分化、組織培養工学、26, 403-406, 2000.

山根利之、林眞一、國貞隆弘、ES細胞分化誘導系とその応用、実験医学、18,456-460, 2000.

山根利之、林眞一、國貞隆弘、ES細胞から色素細胞への分化制御、細胞工学、20, 984-988, 2001.

國貞隆弘、表皮の幹細胞システム、Connective tissue 33, 301-308, 2001.

國貞隆弘、ES細胞からの色素細胞の誘導、遺伝子医学、5, 502-505, 2001.

石崎勝彦、國貞隆弘、ES細胞からの組織様構造の誘導、Molecular Medicine40, 123-127, 2003.

國貞隆弘、平野真理子、川口敦司、本橋力、ES細胞からの眼様構造の誘導、再生医療、259-632003.

國貞隆弘、ヒトES細胞、培養細胞実験ハンドブック、p235-238, 羊土社、2004.

國貞隆弘、青木仁美、本橋力、フィーダー細胞によるES細胞の維持と分化誘導、培養細胞実験ハンドブック、p260-263, 羊土社、2004.

國貞隆弘、整形外科領域の再生医療(第一回基礎領域)、臨床整形外科、39, 1077-1081, 2004.

高橋知之、藤原久義、國貞隆弘、小財健一郎、ES細胞の心筋分化と再生医学への技術開発、最新医学、601688-16942005.

國貞隆弘、ES細胞(万能細胞)の研究の現状、現代医学、549-142006.

青木仁美、國貞隆弘、ES細胞と目の器官形成、アニテックス, 196-142006.

國貞隆弘、再生医療を巡る新たな展開、日本臨床内科医会会誌、22533-5422008.

青木仁美、國貞隆弘、幹細胞を用いた再生医療の可能性、幹細胞の分化誘導と応用、p575-590(株式会社エヌ・ティー・エヌ)2009.

山田泰広、青木仁美、國貞隆弘、原 明、iPS細胞と疾患解析、細胞工学200828巻;228-2312009.

副田明男,國貞隆弘,岩間亨, 幹細胞の光と陰?iPS細胞・ガン幹細胞が脳腫瘍研究を変える, 脳神経外科速報200919巻;1046-10532009.

 

【特許】

2006227日,「フィーダー細胞の非存在下、無血清培地中で胚性幹細胞を浮遊培養することを特徴とする、胚性幹細胞の維持方法」,國貞隆弘,本橋力,岐阜大学,日本,特願2006-051115.

 

2008102日,「効率的な人工多能性幹細胞の樹立方法」,手塚建一,柴田敏之,國貞隆弘,玉置也剛,武田知子,山中伸弥,高橋和利,岐阜大学50%京都大学50%,米・欧州・豪・中国・シンガポール・加・印,PCT/JP 2008/068320.