S-700

JMS-Amsun 200/GI

GC Mate II GC/MS

Room 1

Mass Spectrometry (MS) is one of the fastest-growing areas in analytical instrumentation. This analysis is usually achieved by degrading a few nanogram of a compound and recording the fragmentation pattern derived from the mass fragmentation processes.

The molecules of the volatile sample are ionized and divided into lower molecular mass ions (fragment ions) in the ionization room. The resulting positive charged molecule and its fragment ions are accelerated into magnetic field unit which separates them in space according to their masses, and the detector responds to the arrival of the ions. The record of ion abundance versus mass, which is usually called a mass spectrum, shows a pattern inherent of the molecule analyzed, and therefore, we can identify the molecule and determine the molecular weight and molecular structure of known or unknown compounds. The use of mass spectrometry in support of synthetic, organic, and pharmaceutical chemistry is well established.

The Center has three mass spectrometers and a MS data processing system. Each mass spectrometer is equipped with a gas chromatograph, and one can analyze a series of compounds in a mixed sample without the separation procedures. The main specifications of the mass spectrometers and the data processing system are as follows:

1) JEOL S-700 System, double focus type (Room 1)
Specifications:
Mass range: m/z =10-1000 Da
Resolution: M/DM=2000;
Sensitivity: 1 ng of methyl stearate M ⁺ (298) is detectable (S/N ratio > 100
Sample introduction: Direct injection (DI) and gas chromatograph (GC).
Ionization methods: Electron ionization (EI) and chemical ionization (CI)
Measurement mode: Mass spectrum, mass fragmentgram, total ion chromatogram, mass chromatogram, etc.

2) JEOL JMS-Amsun 200/GI , quadrupole type (Room 1)
Specifications:
Mass range: m/z =1-6000 Da
Resolution: 25,000
Sensitivity: 50 pg of methyl stearate M ⁺ (298) is detectable (S/N ratio > 10).
Sample introduction: Direct injection (DI) and gas chromatograph (GC).
Ionization methods: EI, CI, fast atom bombardment (FAB), and field desorption (FD) method
Measurement mode: Mass spectrum, mass fragmentgram, total ion chromatogram, mass chromatogram, etc.
Others: Measurements of MIKES (daughter ion), metastable ion, negative ions, and millimass are available

3) GC Mate II GC/MS System (JEOL) , quadrupole type (Room 1)
The benchtop double-focusing mass spectrometer is connected with a gas chromatograph. GCmate offers high sensitivity, highly reproducible mass spectra and quantitation, as well as high resolution (up to 5,000 at 10% valley) and accurate mass measurement for determining elemental compositions. The system is fully controlled by a computer with easy-to-use window-based user interface.
Specifications:
Mass range: m/z =1-1000 Da (accelerating voltage: 2.5 kV)
?m/z =1-2000 Da (accelerating voltage: 1.25 kV)
Resolution: 500,1000, 3000, 5000 (changed by computer)
Sensitivity: 30 pg of methyl stearate M ⁺ (298) is detectable (S/N ratio > 10).
Sample introduction: Direct injection (DI) and gas chromatograph (GC).
Ionization methods: EI, CI, fast atom bombardment (FAB), and field desorption (FD) method
Measurement mode: Mass spectrum, mass fragmentgram, total ion chromatogram, mass chromatogram, etc.